NCI2170 Lung Cancer Organoid with Ki67 Cell Proliferation Labeling
|Visikol® HISTO-M™||200 μL|
(PBS / 0.2% Triton™ X-100 / 0.3 M glycine / 20% DMSO)
|10x Washing Buffer
(10x PBS with 2% Tween™ 20 and 100µg/mL heparin)
(PBS/0.2% Triton™ X-100 /6% donkey serum/10% DMSO)
(PBS/0.2% Tween™ 20/Heparin /3% donkey serum/5% DMSO)
|Primary and Secondary Antibodies|
e.g. DAPI, Hoeschst, SYTOX, or TO-PRO stains
Anhydrous (reagent grade)
|Phosphate buffered saline (PBS)||1 mL|
|PBS with 1% Triton™ X-100||400 μL|
Rounded, clear bottom well plate with lid.
We recommend Corning® Cat. 4515.
Warning! Do not treat specimens with other media after clearing. For optimal results, imaging should be performed directly in Visikol HISTO reagents. Visikol HISTO solutions contain anti-fade agents, and specimens cleared with Visikol HISTO do not need to be mounted in an additional anti-fade media. Other solutions (particularly aqueous media) will cloud the tissue or completely reverse the clearing, interfering with 3D volume imaging. Once the cleared tissues have been successfully imaged, see below for additional information on reversing the clearing to perform downstream assays.